The Composition of Cauliflower Mosaic Virus Protein

Nuclear targeting of the cauliflower mosaic virus coat protein.

The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of open reading frame IV, contains an N-terminal nuclear localization signal (NLS). The NLS is exposed on the surface of the virion and is thus avail...

Cauliflower mosaic virus isolate CMV-1.

Biochemical characterization of the helper component of Cauliflower mosaic virus.

The helper component of Cauliflower mosaic virus is encoded by viral gene II. This protein (P2) is dispensable for virus replication but required for aphid transmission. The purification of P2 has never been reported, and hence its biochemical properties are largely unknown. We produced the P2 protein via a recombinant baculovirus with a His tag fused at the N terminus. The fusion protein was p...

A dissection of the cauliflower mosaic virus polyadenylation signal.

Mutagenesis analysis of the polyadenylation [poly(A)] signal from the cauliflower mosaic virus (CaMV), a plant pararetrovirus, revealed striking differences to known vertebrate poly(A) signals. Our results show that (1) the AATAAA sequence is necessary for efficient cleavage at the poly(A) site, although the requirement for an authentic AATAAA might be less stringent in plant than in vertebrate...

The double-stranded DNA of cauliflower mosaic virus.

Examination of the physical structure of cauliflower mosaic virus DNA by electron microscopy and ultracentrifugal analysis showed that the DNA could be isolated mainly as a circular molecule of mol. wt. 4"7 x Io 6. Nearest-neighbour frequency analysis showed that the DNA has a chemical structure which exhibits close similarities to that of the host cauliflower DNA. The relationships of this pla...